Biology

"Space Medicine and the Future of Human Exploration"

A self photo of Dr. Afshin Beheshti in a navy blue collared button up shirt.
Dr. Afshin Beheshti 
 

Bio:
Afshin Beheshti, PhD is a Professor of Surgery and of Computational & Systems Biology at the University of Pittsburgh School. He serves as Director of the newly launched Space Center for Space Biomedicine and as Associate Director of the McGowan Institute for Regenerative Medicine at Pitt​. In addition, Dr. Beheshti holds a visiting scientist appointment at the Broad Institute of MIT and Harvard​.

Abstract:
Human spaceflight presents significant health challenges driven by microgravity, space radiation, isolation, and other environmental stressors. Recent multi-omics research has revealed that mitochondrial dysfunction is a central biological consequence of space travel, contributing to systemic impacts such as accelerated aging, cardiovascular disease, and impaired metabolic function. Data from astronaut missions and ground-based space analogs demonstrate persistent mitochondrial suppression even after returning to Earth. This talk highlights how space serves as a unique accelerated model for studying human diseases and aging, offering insights applicable both to space exploration and terrestrial medicine. Using advanced 3D organoid models and multi-omics analysis, we have identified promising countermeasures, including the natural flavonoid Kaempferol, which restores mitochondrial bioenergetics and reverses radiation-induced gene expression changes in multiple tissues. These findings underscore the critical role of mitochondria as both biomarkers and therapeutic targets for sustaining human health in deep space missions, while also advancing precision medicine strategies on Earth.

Date:
Location:
THM 116

"Space Medicine and the Future of Human Exploration"

A self photo of Dr. Afshin Beheshti in a navy blue collared button up shirt.
Dr. Afshin Beheshti 
 

Bio:
Afshin Beheshti, PhD is a Professor of Surgery and of Computational & Systems Biology at the University of Pittsburgh School. He serves as Director of the newly launched Space Center for Space Biomedicine and as Associate Director of the McGowan Institute for Regenerative Medicine at Pitt​. In addition, Dr. Beheshti holds a visiting scientist appointment at the Broad Institute of MIT and Harvard​.

Abstract:
Human spaceflight presents significant health challenges driven by microgravity, space radiation, isolation, and other environmental stressors. Recent multi-omics research has revealed that mitochondrial dysfunction is a central biological consequence of space travel, contributing to systemic impacts such as accelerated aging, cardiovascular disease, and impaired metabolic function. Data from astronaut missions and ground-based space analogs demonstrate persistent mitochondrial suppression even after returning to Earth. This talk highlights how space serves as a unique accelerated model for studying human diseases and aging, offering insights applicable both to space exploration and terrestrial medicine. Using advanced 3D organoid models and multi-omics analysis, we have identified promising countermeasures, including the natural flavonoid Kaempferol, which restores mitochondrial bioenergetics and reverses radiation-induced gene expression changes in multiple tissues. These findings underscore the critical role of mitochondria as both biomarkers and therapeutic targets for sustaining human health in deep space missions, while also advancing precision medicine strategies on Earth.

Date:
Location:
THM 116

"Understanding the Sources of Regenerative Capacity in Animals"

Dr. Alejandro Sánchez Alvarado standing in front of a white dry erase board with his arms crossed while smiling. The dry erase board behind him has biology graphics in different colors.

Dr. Alejandro Sánchez Alvarado | Sánchez Alvarado Lab

Bio:
Sánchez Alvarado received a BS in molecular biology and chemistry from Vanderbilt University in Nashville, TN, and a PhD in pharmacology and cell biophysics from the University of Cincinnati College of Medicine in Cincinnati, OH. He performed postdoctoral and independent research at the Carnegie Institution of Washington, Department of Embryology in Baltimore, MD. In 2002, he joined the faculty of the University of Utah School of Medicine in Salt Lake City where he held the H.A. & Edna Benning Presidential Endowed Chair. In 2005, he was named a Howard Hughes Medical Institute Investigator. He joined the Stowers Institute for Medical Research in Kansas City in 2011 and became the President and Chief Scientific Officer of the Stowers Institute in 2022. He also holds the Priscilla Wood Neaves Chair in the Biomedical Sciences.

Sánchez Alvarado is an elected member of the National Academy of Science, the American Academy of Arts and Sciences, and the Latin American Academy of Sciences, a Kavli Fellow of the National Academy of Sciences USA, a Fellow of the Marine Biological Laboratory in Woods Hole, MA, a Fellow of the American Association for the Advancement of Science, and a recipient of a National Institutes of Health MERIT award, the EE Just Medal for Scientific Achievement, and the Vilcek Prize in Biomedical Sciences. He has served on numerous scientific advisory committees and boards including the National Advisory Council of the National Institute of General Medical Sciences, National Institutes of Health, and presently serves on the Board of Directors of American Century Investments.

Sánchez Alvarado’s work has the potential to lead to a better understanding of how the adult forms of higher organisms, including humans, carry out their biological functions. His research also has led to insights on the molecular and genetic drivers of both regenerative and degenerative cellular processes that contribute to disease.

Abstract:
It is paradoxical that for many organisms (including humans), the apparent anatomical stability of their adult bodies is maintained by constant change. Despite the importance of tissue homeostasis and regeneration to human biology and health, relatively little is known about how these processes are regulated. As such, numerous questions remain unanswered, including: How do organ systems maintain their order and function while in a state of cell flux? How do animals control and coordinate the size and cell number of multiple organ systems? Does regeneration of body parts lost to injury invoke embryonic processes, generic patterning mechanisms, or unique circuitry comprised of well-established patterning genes? Answering any of these questions would set a baseline from which to try to enhance regenerative properties in multicellular organisms such as humans, particularly after injury.

One way to solve a complex problem is to reduce it to a simpler, easier to answer problem. Therefore, reducing the complexities of regeneration and tissue homeostasis to the study of comparatively simpler systems would allow for a systematic dissection and mechanistic understanding of these processes. Here, I will discuss how the use of single-cell and spatial transcriptomics is helping define the cellular and molecular environments that support pluripotency in the highly regenerative freshwater planarian Schmidtea mediterranea and regeneration of missing organs in the hemichordate Ptychodera flava. Our studies are beginning to shed light on the way adult animals regulate tissue homeostasis and the replacement of body parts lost to injury.

Date:
Location:
THM 116

"Understanding the Sources of Regenerative Capacity in Animals"

Dr. Alejandro Sánchez Alvarado standing in front of a white dry erase board with his arms crossed while smiling. The dry erase board behind him has biology graphics in different colors.

Dr. Alejandro Sánchez Alvarado | Sánchez Alvarado Lab

Bio:
Sánchez Alvarado received a BS in molecular biology and chemistry from Vanderbilt University in Nashville, TN, and a PhD in pharmacology and cell biophysics from the University of Cincinnati College of Medicine in Cincinnati, OH. He performed postdoctoral and independent research at the Carnegie Institution of Washington, Department of Embryology in Baltimore, MD. In 2002, he joined the faculty of the University of Utah School of Medicine in Salt Lake City where he held the H.A. & Edna Benning Presidential Endowed Chair. In 2005, he was named a Howard Hughes Medical Institute Investigator. He joined the Stowers Institute for Medical Research in Kansas City in 2011 and became the President and Chief Scientific Officer of the Stowers Institute in 2022. He also holds the Priscilla Wood Neaves Chair in the Biomedical Sciences.

Sánchez Alvarado is an elected member of the National Academy of Science, the American Academy of Arts and Sciences, and the Latin American Academy of Sciences, a Kavli Fellow of the National Academy of Sciences USA, a Fellow of the Marine Biological Laboratory in Woods Hole, MA, a Fellow of the American Association for the Advancement of Science, and a recipient of a National Institutes of Health MERIT award, the EE Just Medal for Scientific Achievement, and the Vilcek Prize in Biomedical Sciences. He has served on numerous scientific advisory committees and boards including the National Advisory Council of the National Institute of General Medical Sciences, National Institutes of Health, and presently serves on the Board of Directors of American Century Investments.

Sánchez Alvarado’s work has the potential to lead to a better understanding of how the adult forms of higher organisms, including humans, carry out their biological functions. His research also has led to insights on the molecular and genetic drivers of both regenerative and degenerative cellular processes that contribute to disease.

Abstract:
It is paradoxical that for many organisms (including humans), the apparent anatomical stability of their adult bodies is maintained by constant change. Despite the importance of tissue homeostasis and regeneration to human biology and health, relatively little is known about how these processes are regulated. As such, numerous questions remain unanswered, including: How do organ systems maintain their order and function while in a state of cell flux? How do animals control and coordinate the size and cell number of multiple organ systems? Does regeneration of body parts lost to injury invoke embryonic processes, generic patterning mechanisms, or unique circuitry comprised of well-established patterning genes? Answering any of these questions would set a baseline from which to try to enhance regenerative properties in multicellular organisms such as humans, particularly after injury.

One way to solve a complex problem is to reduce it to a simpler, easier to answer problem. Therefore, reducing the complexities of regeneration and tissue homeostasis to the study of comparatively simpler systems would allow for a systematic dissection and mechanistic understanding of these processes. Here, I will discuss how the use of single-cell and spatial transcriptomics is helping define the cellular and molecular environments that support pluripotency in the highly regenerative freshwater planarian Schmidtea mediterranea and regeneration of missing organs in the hemichordate Ptychodera flava. Our studies are beginning to shed light on the way adult animals regulate tissue homeostasis and the replacement of body parts lost to injury.

Date:
Location:
THM 116

"Understanding the Sources of Regenerative Capacity in Animals"

Dr. Alejandro Sánchez Alvarado standing in front of a white dry erase board with his arms crossed while smiling. The dry erase board behind him has biology graphics in different colors.

Dr. Alejandro Sánchez Alvarado | Sánchez Alvarado Lab

Bio:
Sánchez Alvarado received a BS in molecular biology and chemistry from Vanderbilt University in Nashville, TN, and a PhD in pharmacology and cell biophysics from the University of Cincinnati College of Medicine in Cincinnati, OH. He performed postdoctoral and independent research at the Carnegie Institution of Washington, Department of Embryology in Baltimore, MD. In 2002, he joined the faculty of the University of Utah School of Medicine in Salt Lake City where he held the H.A. & Edna Benning Presidential Endowed Chair. In 2005, he was named a Howard Hughes Medical Institute Investigator. He joined the Stowers Institute for Medical Research in Kansas City in 2011 and became the President and Chief Scientific Officer of the Stowers Institute in 2022. He also holds the Priscilla Wood Neaves Chair in the Biomedical Sciences.

Sánchez Alvarado is an elected member of the National Academy of Science, the American Academy of Arts and Sciences, and the Latin American Academy of Sciences, a Kavli Fellow of the National Academy of Sciences USA, a Fellow of the Marine Biological Laboratory in Woods Hole, MA, a Fellow of the American Association for the Advancement of Science, and a recipient of a National Institutes of Health MERIT award, the EE Just Medal for Scientific Achievement, and the Vilcek Prize in Biomedical Sciences. He has served on numerous scientific advisory committees and boards including the National Advisory Council of the National Institute of General Medical Sciences, National Institutes of Health, and presently serves on the Board of Directors of American Century Investments.

Sánchez Alvarado’s work has the potential to lead to a better understanding of how the adult forms of higher organisms, including humans, carry out their biological functions. His research also has led to insights on the molecular and genetic drivers of both regenerative and degenerative cellular processes that contribute to disease.

Abstract:
It is paradoxical that for many organisms (including humans), the apparent anatomical stability of their adult bodies is maintained by constant change. Despite the importance of tissue homeostasis and regeneration to human biology and health, relatively little is known about how these processes are regulated. As such, numerous questions remain unanswered, including: How do organ systems maintain their order and function while in a state of cell flux? How do animals control and coordinate the size and cell number of multiple organ systems? Does regeneration of body parts lost to injury invoke embryonic processes, generic patterning mechanisms, or unique circuitry comprised of well-established patterning genes? Answering any of these questions would set a baseline from which to try to enhance regenerative properties in multicellular organisms such as humans, particularly after injury.

One way to solve a complex problem is to reduce it to a simpler, easier to answer problem. Therefore, reducing the complexities of regeneration and tissue homeostasis to the study of comparatively simpler systems would allow for a systematic dissection and mechanistic understanding of these processes. Here, I will discuss how the use of single-cell and spatial transcriptomics is helping define the cellular and molecular environments that support pluripotency in the highly regenerative freshwater planarian Schmidtea mediterranea and regeneration of missing organs in the hemichordate Ptychodera flava. Our studies are beginning to shed light on the way adult animals regulate tissue homeostasis and the replacement of body parts lost to injury.

Date:
Location:
THM 116

"Understanding the Sources of Regenerative Capacity in Animals"

Dr. Alejandro Sánchez Alvarado standing in front of a white dry erase board with his arms crossed while smiling. The dry erase board behind him has biology graphics in different colors.

Dr. Alejandro Sánchez Alvarado | Sánchez Alvarado Lab

Bio:
Sánchez Alvarado received a BS in molecular biology and chemistry from Vanderbilt University in Nashville, TN, and a PhD in pharmacology and cell biophysics from the University of Cincinnati College of Medicine in Cincinnati, OH. He performed postdoctoral and independent research at the Carnegie Institution of Washington, Department of Embryology in Baltimore, MD. In 2002, he joined the faculty of the University of Utah School of Medicine in Salt Lake City where he held the H.A. & Edna Benning Presidential Endowed Chair. In 2005, he was named a Howard Hughes Medical Institute Investigator. He joined the Stowers Institute for Medical Research in Kansas City in 2011 and became the President and Chief Scientific Officer of the Stowers Institute in 2022. He also holds the Priscilla Wood Neaves Chair in the Biomedical Sciences.

Sánchez Alvarado is an elected member of the National Academy of Science, the American Academy of Arts and Sciences, and the Latin American Academy of Sciences, a Kavli Fellow of the National Academy of Sciences USA, a Fellow of the Marine Biological Laboratory in Woods Hole, MA, a Fellow of the American Association for the Advancement of Science, and a recipient of a National Institutes of Health MERIT award, the EE Just Medal for Scientific Achievement, and the Vilcek Prize in Biomedical Sciences. He has served on numerous scientific advisory committees and boards including the National Advisory Council of the National Institute of General Medical Sciences, National Institutes of Health, and presently serves on the Board of Directors of American Century Investments.

Sánchez Alvarado’s work has the potential to lead to a better understanding of how the adult forms of higher organisms, including humans, carry out their biological functions. His research also has led to insights on the molecular and genetic drivers of both regenerative and degenerative cellular processes that contribute to disease.

Abstract:
It is paradoxical that for many organisms (including humans), the apparent anatomical stability of their adult bodies is maintained by constant change. Despite the importance of tissue homeostasis and regeneration to human biology and health, relatively little is known about how these processes are regulated. As such, numerous questions remain unanswered, including: How do organ systems maintain their order and function while in a state of cell flux? How do animals control and coordinate the size and cell number of multiple organ systems? Does regeneration of body parts lost to injury invoke embryonic processes, generic patterning mechanisms, or unique circuitry comprised of well-established patterning genes? Answering any of these questions would set a baseline from which to try to enhance regenerative properties in multicellular organisms such as humans, particularly after injury.

One way to solve a complex problem is to reduce it to a simpler, easier to answer problem. Therefore, reducing the complexities of regeneration and tissue homeostasis to the study of comparatively simpler systems would allow for a systematic dissection and mechanistic understanding of these processes. Here, I will discuss how the use of single-cell and spatial transcriptomics is helping define the cellular and molecular environments that support pluripotency in the highly regenerative freshwater planarian Schmidtea mediterranea and regeneration of missing organs in the hemichordate Ptychodera flava. Our studies are beginning to shed light on the way adult animals regulate tissue homeostasis and the replacement of body parts lost to injury.

Date:
Location:
THM 116

"Orchestrating Mammalian Tissue Healing at the Organ Scale"

Image of Dr. Yvon WoappiDr. Yvon Woappi | Woappi Lab

Bio:
Dr. Yvon Woappi is the Herbert and Florence Irving Assistant Professor of Physiology and Cellular Biophysics, Dermatology, and Biomedical Engineering at Columbia University. His research leverages gene editing and multiomic technologies to uncover how autonomous multicellular orchestration facilitates deep wound repair – a process critical to many conditions including diabetic ulcers and carcinomas. Dr. Woappi earned his Ph.D. as a Grace Jordan McFadden Fellow at the University of South Carolina and completed his postdoctoral training in the Harvard Dermatology Research Training Program at Harvard Medical School. Dr. Woappi’s pioneering early-career research is laying the foundation for synthetic wound regeneration, a systems bioengineering approach that leverages cellular heterogeneity to enhance tissue regeneration.

Abstract:
As the organ most frequently exposed to predatory pressures, the integument has acquired broad functions, including camouflage, thermoregulation, sensory perception, and tissue repair. These roles are executed through a complex interplay of tissue substructures, including several mini-organ appendages (hair follicles, sebaceous glands, arrector pili muscle, and assorted pilosebaceous units) and five central adnexal structures (blood vessels, sensory neurons, collagenous tissues, immune components, and deep fascia), all embedded within three superimposed tissue strata (the epidermis, dermis, and hypodermis). Given this intricate architecture, the healing of deep skin wounds requires a coordinated organ-level response involving varied cell populations originating from virtually all three embryonic germ layers—ectoderm, mesoderm, and endoderm. However, a comprehensive understanding of the cellular and molecular logic orchestrating this crosstissue response in mammals remains incomplete. Here, we present the Organ-Scale Wound Healing Atlases (OWHA), a comprehensive multiomic single-cell and spatial transcriptomic dataset that captures the dynamic microanatomical tissue niches of the mammalian integument during the entire wound healing sequence, including early and late healing phases. By incorporating multi-omics data across all major phases of healing, OWHA uncovered novel emergent healing cell states and their coordinated cell fate decisions uniquely (multilineage crosstalk) executed after injury, and delineated critical tissue trajectories required for eKective healing of deep wounds. Importantly, comparative analysis between human and mouse revealed conserved network between the epithelial and neuro-endothelial vasculature....(Add missing groups in human only found in our multi modal approach) By providing deeper mechanistic insights of mammalian tissue adaptations for injury response, OWHA serves as a valuable resource for understanding the cellular and molecular mechanisms underlying wound healing in the mammalian integument.

A picture of mouse skin with hair follicles.

Date:
Location:
THM 116

"Orchestrating Mammalian Tissue Healing at the Organ Scale"

Image of Dr. Yvon WoappiDr. Yvon Woappi | Woappi Lab

Bio:
Dr. Yvon Woappi is the Herbert and Florence Irving Assistant Professor of Physiology and Cellular Biophysics, Dermatology, and Biomedical Engineering at Columbia University. His research leverages gene editing and multiomic technologies to uncover how autonomous multicellular orchestration facilitates deep wound repair – a process critical to many conditions including diabetic ulcers and carcinomas. Dr. Woappi earned his Ph.D. as a Grace Jordan McFadden Fellow at the University of South Carolina and completed his postdoctoral training in the Harvard Dermatology Research Training Program at Harvard Medical School. Dr. Woappi’s pioneering early-career research is laying the foundation for synthetic wound regeneration, a systems bioengineering approach that leverages cellular heterogeneity to enhance tissue regeneration.

Abstract:
As the organ most frequently exposed to predatory pressures, the integument has acquired broad functions, including camouflage, thermoregulation, sensory perception, and tissue repair. These roles are executed through a complex interplay of tissue substructures, including several mini-organ appendages (hair follicles, sebaceous glands, arrector pili muscle, and assorted pilosebaceous units) and five central adnexal structures (blood vessels, sensory neurons, collagenous tissues, immune components, and deep fascia), all embedded within three superimposed tissue strata (the epidermis, dermis, and hypodermis). Given this intricate architecture, the healing of deep skin wounds requires a coordinated organ-level response involving varied cell populations originating from virtually all three embryonic germ layers—ectoderm, mesoderm, and endoderm. However, a comprehensive understanding of the cellular and molecular logic orchestrating this crosstissue response in mammals remains incomplete. Here, we present the Organ-Scale Wound Healing Atlases (OWHA), a comprehensive multiomic single-cell and spatial transcriptomic dataset that captures the dynamic microanatomical tissue niches of the mammalian integument during the entire wound healing sequence, including early and late healing phases. By incorporating multi-omics data across all major phases of healing, OWHA uncovered novel emergent healing cell states and their coordinated cell fate decisions uniquely (multilineage crosstalk) executed after injury, and delineated critical tissue trajectories required for eKective healing of deep wounds. Importantly, comparative analysis between human and mouse revealed conserved network between the epithelial and neuro-endothelial vasculature....(Add missing groups in human only found in our multi modal approach) By providing deeper mechanistic insights of mammalian tissue adaptations for injury response, OWHA serves as a valuable resource for understanding the cellular and molecular mechanisms underlying wound healing in the mammalian integument.

A picture of mouse skin with hair follicles.

Date:
Location:
THM 116

"Orchestrating Mammalian Tissue Healing at the Organ Scale"

Image of Dr. Yvon WoappiDr. Yvon Woappi | Woappi Lab

Bio:
Dr. Yvon Woappi is the Herbert and Florence Irving Assistant Professor of Physiology and Cellular Biophysics, Dermatology, and Biomedical Engineering at Columbia University. His research leverages gene editing and multiomic technologies to uncover how autonomous multicellular orchestration facilitates deep wound repair – a process critical to many conditions including diabetic ulcers and carcinomas. Dr. Woappi earned his Ph.D. as a Grace Jordan McFadden Fellow at the University of South Carolina and completed his postdoctoral training in the Harvard Dermatology Research Training Program at Harvard Medical School. Dr. Woappi’s pioneering early-career research is laying the foundation for synthetic wound regeneration, a systems bioengineering approach that leverages cellular heterogeneity to enhance tissue regeneration.

Abstract:
As the organ most frequently exposed to predatory pressures, the integument has acquired broad functions, including camouflage, thermoregulation, sensory perception, and tissue repair. These roles are executed through a complex interplay of tissue substructures, including several mini-organ appendages (hair follicles, sebaceous glands, arrector pili muscle, and assorted pilosebaceous units) and five central adnexal structures (blood vessels, sensory neurons, collagenous tissues, immune components, and deep fascia), all embedded within three superimposed tissue strata (the epidermis, dermis, and hypodermis). Given this intricate architecture, the healing of deep skin wounds requires a coordinated organ-level response involving varied cell populations originating from virtually all three embryonic germ layers—ectoderm, mesoderm, and endoderm. However, a comprehensive understanding of the cellular and molecular logic orchestrating this crosstissue response in mammals remains incomplete. Here, we present the Organ-Scale Wound Healing Atlases (OWHA), a comprehensive multiomic single-cell and spatial transcriptomic dataset that captures the dynamic microanatomical tissue niches of the mammalian integument during the entire wound healing sequence, including early and late healing phases. By incorporating multi-omics data across all major phases of healing, OWHA uncovered novel emergent healing cell states and their coordinated cell fate decisions uniquely (multilineage crosstalk) executed after injury, and delineated critical tissue trajectories required for eKective healing of deep wounds. Importantly, comparative analysis between human and mouse revealed conserved network between the epithelial and neuro-endothelial vasculature....(Add missing groups in human only found in our multi modal approach) By providing deeper mechanistic insights of mammalian tissue adaptations for injury response, OWHA serves as a valuable resource for understanding the cellular and molecular mechanisms underlying wound healing in the mammalian integument.

A picture of mouse skin with hair follicles.

Date:
Location:
THM 116

"Orchestrating Mammalian Tissue Healing at the Organ Scale"

Image of Dr. Yvon WoappiDr. Yvon Woappi | Woappi Lab

Bio:
Dr. Yvon Woappi is the Herbert and Florence Irving Assistant Professor of Physiology and Cellular Biophysics, Dermatology, and Biomedical Engineering at Columbia University. His research leverages gene editing and multiomic technologies to uncover how autonomous multicellular orchestration facilitates deep wound repair – a process critical to many conditions including diabetic ulcers and carcinomas. Dr. Woappi earned his Ph.D. as a Grace Jordan McFadden Fellow at the University of South Carolina and completed his postdoctoral training in the Harvard Dermatology Research Training Program at Harvard Medical School. Dr. Woappi’s pioneering early-career research is laying the foundation for synthetic wound regeneration, a systems bioengineering approach that leverages cellular heterogeneity to enhance tissue regeneration.

Abstract:
As the organ most frequently exposed to predatory pressures, the integument has acquired broad functions, including camouflage, thermoregulation, sensory perception, and tissue repair. These roles are executed through a complex interplay of tissue substructures, including several mini-organ appendages (hair follicles, sebaceous glands, arrector pili muscle, and assorted pilosebaceous units) and five central adnexal structures (blood vessels, sensory neurons, collagenous tissues, immune components, and deep fascia), all embedded within three superimposed tissue strata (the epidermis, dermis, and hypodermis). Given this intricate architecture, the healing of deep skin wounds requires a coordinated organ-level response involving varied cell populations originating from virtually all three embryonic germ layers—ectoderm, mesoderm, and endoderm. However, a comprehensive understanding of the cellular and molecular logic orchestrating this crosstissue response in mammals remains incomplete. Here, we present the Organ-Scale Wound Healing Atlases (OWHA), a comprehensive multiomic single-cell and spatial transcriptomic dataset that captures the dynamic microanatomical tissue niches of the mammalian integument during the entire wound healing sequence, including early and late healing phases. By incorporating multi-omics data across all major phases of healing, OWHA uncovered novel emergent healing cell states and their coordinated cell fate decisions uniquely (multilineage crosstalk) executed after injury, and delineated critical tissue trajectories required for eKective healing of deep wounds. Importantly, comparative analysis between human and mouse revealed conserved network between the epithelial and neuro-endothelial vasculature....(Add missing groups in human only found in our multi modal approach) By providing deeper mechanistic insights of mammalian tissue adaptations for injury response, OWHA serves as a valuable resource for understanding the cellular and molecular mechanisms underlying wound healing in the mammalian integument.

A picture of mouse skin with hair follicles.

Date:
Location:
THM 116